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KMID : 0861020170320060009
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Korea Journal of Herbology
2017 Volume.32 No. 6 p.9 ~ p.15
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Inhibitory Effects of Scrophulariae Radix on β-hexosaminidase release and cytokine production in RBL-2H3 cells
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Kim Se-Gie
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Abstract
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Objectives : Traditional medicines isolated from natural products often have positive effects in the prevention and healing of various immune disorders, such as allergy and atopic inflammation. Scrophulariae Radix (SR) been used in oriental medicine used for treatment of acute and chronic inflammatory diseases. Mast cells are known to play important roles in the initiation of allergic reactions. In this study, we investigated the effects of SR ethanol extract on inflammatory responses in IgE-stimulated RBL-2H3 mast cells.
Methods : Rat basophilic leukemia RBL-2H3 cells were purchased from Korean Cell Line Bank (KCLB No. 22256). Cell viability was measured by MTT assay. Assays for β-Hexosaminidase Secretion : RBL-2H3 cells were sensitized with dinitrophenyl-ImmunoglobulinE (DNP IgE). The next antigen DNP-BSA (25 ng / ㎖) was added for 10 minutes and the reaction was terminated after 5 minutes in the ice bath. To determine β-hexosaminidase release, supernatants were aliquoted into 96-well plates. Samples were mixed with substrate solution and incubated for 1 h at 37 ℃. Absorbance was measured with a spectrophotometer at 405 ㎚. IL-4 and tumor necrosis factor-α (TNF-α) concentrations in cell culture supernatants were measured using enzyme-linked immunosorbent assay (ELISA) kits.
Results : The cytotoxicity of SRE in RBL-2H3 cells was less than 5%. SRE inhibited DNP-IgE-imduced degranulation of mast cells in RBL-2H3 cells. Also significantly decreased the levels of inflammatory cytokine, IL-4 and TNF-alpha. In this study, the SRE showed potential anti-allergic and antiinflammatory.
Conclusions : These results indicate that SRE could be inhibit the allergic response through suppressing the mast cell activation.
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KEYWORD
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Scrophulariae Radix, anti-allergic effect, RBL-2H3 mast cell, inflammatory reactions, β-hexosaminidase, IL-4, TNF-alpha
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